antibodies against β3 tubulin  (Cell Signaling Technology Inc)

Journal: Molecules

Article Title: Colchicine Binding Site Tubulin Inhibitors Impair Vincristine-Resistant Neuroblastoma Cell Function

doi: 10.3390/molecules30102186

Figure Lengend Snippet: Beta3-tubulin levels do not correlate with MYCN amplification status or response to compound treatments. ( a ) Western blots of protein lysates collected from SK-N-AS and Kelly cells compared to lysates from vincristine (VCR)-resistant cells. GAPDH is included as a loading control for comparisons between β-TUBULIN and β3-TUBULIN. ( b ) Western blots of protein lysates from indicated cell lines with varying MYCN amplification status. SH-EP, SH-SY5Y, SK-N-AS, and SK-N-SH are non- MYCN -amplified, while CHP-134, Be(2)C, and Kelly are MYCN -amplified. ( c ) Western blots of protein lysates collected from parental and vincristine-resistant SK-N-AS and Kelly cells showing ABC transporter protein expression. Uncropped Western blots used in this figure are included within the .

Article Snippet: The antibodies used were as follows: c-MYC (Abcam, Cambridge, UK, AB32072), N-MYC (Cell Signaling, Waltham, MA, USA, 51705), GAPDH-HRP (Cell Signaling, 8884), p-S10-H3 (Cell Signaling, 53348), β-TUBULIN (Cell Signaling, 2128), β3-TUBULIN (Cell Signaling, 5568), cleaved PARP (Cell Signaling, 9541), CYCLIN B1 (Cell Signaling, 4138), ABCB1 (Cell Signaling, 13342), and ABCC1 (Cell Signaling, 72202).

Techniques: Amplification, Western Blot, Control, Expressing